Monocyte nonspecific esterase. Enzymologic characterization of a neutral serine esterase associated with myeloid cells.
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چکیده
منابع مشابه
Enzymologic classification of acute leukemias: nonspecific esterase markers distinguish myeloid and lymphoid varieties.
Nonspecific esterase zymograms of purified leukemic cells from a case of acute myelomonocytic leukemia (AMML) and from a case of acute non-B, non-T lymphocytic leukemia (ALL) were produced by isoelectric focusing and staining with alpha-naphthyl acetate (alpha NA) or alpha-naphthyl butyrate (alpha NB) substrate. A "myeloid" zymogram was found with AMML cells, which closely matched control monoc...
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Monocyte nonspecific esterase has been purified from cultured cells of the acute myeloid leukemia cell line. ML-l. The purified enzyme shows the characteristic properties of the monocyte neutral serine carboxyI esterase, with high sensitivity to organophosphorus inhibitors and sodium fluoride inhibitor. The enzyme is a membrane protein which in the native state exists as a monomer of a mol wt o...
متن کاملMonocyte nonspecific esterase: purification and subunit structure.
Monocyte nonspecific esterase has been purified from cultured cells of the acute myeloid leukemia cell line, ML-1. The purified enzyme shows the characteristic properties of the monocyte neutral serine carboxyl esterase, with high sensitivity to organophosphorus inhibitors and sodium fluoride inhibitor. The enzyme is a membrane protein which in the native state exists as a monomer of a mol wt o...
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Several products of CTL and NK cells, including a pore-forming protein (PFP;t perforin or cytolysin) (1-4) and several serine esterases (SE) (5-12), have been cloned and sequenced . These proteins are localized to cytoplasmic granules and represent the known structural components ofthe pore-formation and granule exocytosis models for cell-mediated killing (13-17). Granule mediators are expresse...
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Organop iosphat, insecticides, such as Vapona, Naled, and Rabon, are highly potent inhibitors of an enzyme found in human monocytes. The enzyme, a specific monocyte esterase, could be inhibited by Vapona in blood samples via airborne contamination at levels easily achieved from commercial slow-release insecticide strips. Fifty percent inhibition (150)-as measured on the Hemalog D ( Technicon Co...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1986
ISSN: 0022-1554,1551-5044
DOI: 10.1177/34.6.3457861